|
KMID : 0894520070110010021
|
|
Development & Reproduction
2007 Volume.11 No. 1 p.21 ~ p.29
|
|
|
Expression of Progesterone Receptor Membrane Component 1 and 2 in the Mouse Gonads and Embryos
|
|
Kim Kyeoung-Hwa
Lee Kyung-Ah
|
|
|
Abstract
|
|
|
|
Previously, we found progesterone receptor membrane component 2 (pgrmc2) was highly expressed in germinal vesicle (GV) stage oocytes. The present study was conducted to characterize the expression of pgrmc2, as well as pgrmc1, in the mouse gonads and embryos according to their developmental stages. We found that these membrane components were expressed in ovaries, testes, and embryos at various developmental stages in addition to oocytes. Progesterone-3-O-carboxymethyl oxime-BSA-fluorescein isothiocyanate (P4-BSA-FITC) was applied to visualize the presence of the progesterone receptor on mouse oocyte membrane, and we confirmed that immobilized progesterone is localized at surface of the oocyte. This is, at our knowledge, the first report regarding the expression of membrane component of progesterone receptor in the mouse oocytes, embryos, and gonads. The function and signal transduction pathway of progesterone receptor membrane components in oocytes requires further studies.
|
|
|
KEYWORD
|
|
|
Progesterone receptor, Steroid receptor membrane component, P4-BSA-FITC, Mouse, Oocytes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|